WordNet island%1:06:00:: and FrameNet N/A [Hide]
- In contrast to exon and CpG island density, GC content shows limited variation - in the range 35-55% for most 1 Mb intervals. [W1,I1]: 1.00
- Oocytes were transferred into tubes containing Hepes-buffered TCM-199 (Gibco BRL, Grand Island, NY) supplemented with 0.57 mM cysteine, 10 ng/ml epidermal growth factor (Sigma), 100 IU/ml penicillin, 100 μg/ml streptomycin, 0.25 ng/ml amphotericin, 0.01 IU/ml porcine FSH (Sioux Biochemicals, Sioux Center, IA), 0.01 IU/ml porcine LH (Sioux Biochemicals) and 0.1% (w:v) polyvinyl alcohol (maturation medium) [ 50], and shipped to the laboratory overnight (20-24 h) at 38. [W1,I1]: 1.00
- For each PCR run, a master mixture was prepared on ice with 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 1.5 mM MgCl 2 , 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each primer, 2.5 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA). [W1,I1]: 1.00