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- Some of these activities include nucleic acid binding [ 11 ] , ribonuclease activity (for processing preribosomal RNA) [ 12 ] and association with maturing preribosomal ribonucleoprotein particles [ 13 14 ] . It may also be involved in the transport of ribosomal or other nucleosomal proteins across the nuclear membrane, as it is known to shuttle between the cytoplasm and nucleus and to stimulate the nuclear importation of proteins [ 15 16 ] . Nucleophosmin also appears to be intimately involved in centrosome duplication. [W1,W2,W3,W4,I1]: 1.00
- Candidate mature miRNA sequences were defined by the bounds of the perfectly conserved sequence. [W1,W2,W3,W4,W5,I1]: 1.00
- The scale of the pattern is set by the lengths to which mature macroflbers grow. [W1,W2,W3,W4,I1]: 1.00
- The fragments shown are typical of those produced by toothpick transfer of a mature fiber into fresh medium. [W1,W2,W3,W4,W5,I1]: 1.00
- It was also surprising that the extracellular portion of any of the EGF receptor proteins was also not represented among the proteins found to interact with the extracellular domain of proHB-EGF, since the mature protein proteolytically cleaved from proHB-EGF (mHB-EGF) as well as the membrane bound form are known to interact with the EGF receptor. [W1,W2,W3,W4,I1]: 1.00
- The good news is that they are prime candidates for high-technology management--they only have to get older, not more mature. [W1,W2,W3,W4,I1]: 1.00
- Western blot using anti-26S proteasome was also carried out to show that only Pro-N3-F, not mature N3, nor other proteasome components, was co-precipitated with Smad1 (data not shown). [W1,W2,W3,W4,I1]: 1.00
- This may be related to decreased DNA synthesis in mature confluent HUVEC. [W1,W2,W3,W4,I1]: 1.00
- In the presence of either atorvastatin or simvastatin, the time course of maturation and release perfectly paralleled that observed in the absence of either drug, except that the fractional content of cellular mature holoAPP was approximately 2-fold greater in the presence of drug (i.e. [W1,W2,W3,W4,W5,I1]: 1.00
- The newly forming fiber cell continues to differentiate in the cortex until a mature fiber cell devoid of organelles with suture formation at the ends of the cell is formed. [W1,W2,W3,W4,I1]: 1.00
- The adult testis is a complex organ consisting of numerous different somatic cell types as well as germ cells at all stages of spermatogenesis from the gonocyte stem cells to the mature sperm cells [ 18 ] . Consequently, several unique gene populations, including those involved in the regulation of meiosis, as well as those specific to the various testicular cell types, are expressed in the testis. [W1,W2,W3,W4,W5,I1]: 1.00
- Recombinant human proteins (mature TGFβ 1 , TNF-α, Fibronectin and Laminin 1) were purchased from R&D Systems. [W1,W2,W3,W4,W5,I1]: 1.00
- In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 . [W1,W2,W3,W4,W5,I1]: 1.00
- Recombinant mature TGFβ 1 does not contain a RGD motif, and thus binding of TGFβ 1 to the α V β 6 integrin and the subsequent activation of this integrin must rely on a novel motif distinct from RGD. [W1,W2,W3,W4,I1]: 1.00
- Gene transfer into developing and mature primary T cells has been limited by the lack of efficient techniques for ex vivo or in vivo transfection or transduction. [W1,W2,W3,W4,I1]: 1.00
- But sometimes, at midlife, the grains start to stack up as if the entire pile is still pushing upward, still searching for its mature shape. [W1,W2,W3,W4,I1]: 1.00
- Text mining is ready to deliver handling of complex terminology and nomenclature as a mature service. [W1,W2,W3,W4,W5,I1]: 1.00
- Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml. [W1,W2,W3,W4,W5,I1]: 1.00
- Nonetheless, it is clear that the selective pressure on the non-miRNA-encoding arm is mild, and that it diverges long before the sequence of the mature miRNA does. [W1,W2,W3,W4,I1]: 1.00
- This system produces mature plants in four to six weeks instead of the several months required under ordinary growing conditions. [W1,W2,W3,W4,I1]: 1.00
- This entire process from epithelial cell to mature fiber cell is defined as lens differentiation. [W1,W2,W3,W4,I1]: 1.00
- Prudie thinks, overall, that if this is the young lad's only negative, you should learn to roll with the punches (or burgers, in your case) and hope that his maturing will ameliorate your problem. [W1,W2,W3,W4,W5,I1]: 1.00
- In a mature microfluidic device with push-button operation, results should be available in less than 10 min, and thus can be used to make real-time clinical decisions at the point of care. [W1,W2,W3,W4,W5,I1]: 1.00
- This suggests that either the duration of hypoxic exposure or exposure from neonatal life provided additional physiologic stress that was not seen in more mature animals [ 9]. [W1,W2,W3,W4,I1]: 1.00
- (2004) present data linking Msx1 function to microtubule disassembly during the process of salamander myofiber cellularization and fragmentation (myofibers are formed from myotubes and represent the completely mature form of the differentiated skeletal muscle cell). [W1,W2,W3,W4,W5,I1]: 1.00
- you're going to you're going to pay for uh technology that's not mature yet [W1,W2,W3,W4,I1]: 1.00
- The 81-year-old author's latest is admired as a "mature work": same old Bellovian subject (Jewish man brooding over a woman), but a more subdued, elegiac tone. [W1,W2,W3,W4,I1]: 1.00
- In porcine ovaries, LHR expression was detected in granulosa and theca cells of preovulatory follicles, but not in granulosa lutein cells of the mature CL [ 27 ] . In human ovaries, LHR expression was also detected in granulosa and theca cells of preovulatory follicles, but mature CL showed strong expression in luteal cells, which disappeared during luteal regression. [W1,W2,W3,W4,W5,I1]: 1.00
- [ 12 ] , SMI-31 was described as labeling nuclear epitopes in cells in culture and in neurons of mature rat brain as visualized by cryo-sectioning and confocal microscopy. [W1,W2,W3,W4,I1]: 1.00
- Australia's Sydney Morning Herald presented the weekend's events as "the logical conclusion to the late-20 th century drama which has been quietly corroding the old certainties of political power throughout the world's mature democracies. [W1,W2,W3,W4,I1]: 1.00
- The pulmonary hypertension in mice exposed to hypoxia from birth was more severe than we had observed in previous studies of 4 weeks of hypoxia in mature animals [ 9]. [W1,W2,W3,W4,I1]: 1.00
- His life story enhances this image of fragile purity: He's a late bloomer who didn't publish a mature work until his 50s and didn't become famous until his 60s. [W1,W2,W3,W4,I1]: 1.00
- Although the biological mechanisms underlying the inverse association between menarcheal age and adult obesity are uncertain, it has been suggested that early maturing girls may have a longer period of positive energy balance [ 1 ] , or that various endocrine factors influence both the rate of sexual maturation and the accumulation of body fat [ 6 ] . However, it is also possible that the apparent influence of menarcheal age on adult obesity reflects the underlying importance of childhood obesity, with relatively fat children at increased risk for both early menarche [ 7 9 13 14 15 ] and adult obesity [ 7 16 ] . [W1,W2,W3,W4,I1]: 1.00
- What's interesting here isn't Maslin's personal life but the fact that her youthful writing on Joni Mitchell, say, or Elvis Costello carries more conviction, and therefore more weight, than her mature work on the movies. [W1,W2,W3,W4,I1]: 1.00
- Thus in our transgenic animals, aberrant Wnt activation appears to be limited to mature, postmitotic neurons. [W1,W2,W3,W4,W5,I1]: 1.00
- Cells were stimulated with 10 nM of mature TGFβ 1 or with fibronectin as described below. [W1,W2,W3,W4,I1]: 1.00
- The cultures studied were inoculated by sterile toothpick transfer from mature fibers grown in the same medium. [W1,W2,W3,W4,I1]: 1.00
- The surviving sequences (as few as 5% of the germline sequences) reside on many small MAC chromosomes, which are then highly amplified to the level of the mature MAC. [W1,W2,W3,W4,W5,I1]: 1.00
- It has been suggested that oogonia which have matured to oocytes are less susceptible to degeneration as pregnancy advances [ 2 ] . A prominent role for the aromatic hydrocarbon receptor (e.g. [W1,W2,W3,W4,W5,I1]: 1.00
- To determine an association of ERβ and ERα immunoreactivity with differentiation of mononucleated trophoblast cells into syncytial aggregates, we investigated isolated cytotrophoblast cells and young and mature syncytial aggregates in tissue cultures. [W1,W2,W3,W4,I1]: 1.00
- This Vg1 localization element (VLE) is sufficient for the migration and clustering of Vg1 mRNA to the vegetal cortex of mature oocyte. [W1,W2,W3,W4,I1]: 1.00
- In addition peripheral fibers cease to function as a culture ages consequently a very late arising structure that happens into an exclusion zone can develop there into a mature structure. [W1,W2,W3,W4,I1]: 1.00
- Only later, with the benefit of Speaker Newt Gingrich's GOP Convention instruction (see above), did I come to realize that what we had here was no less than the fulsome gift from a mature Freedom to the island's youth. [W1,W2,W3,W4,W5,I1]: 1.00
- The world has already praised the Brothers as creators of clever, catchy dance tracks, but Surrender will finally make the public respect these guys as mature, intelligent and enterprising musicians" (M. [W1,W2,W3,W4,I1]: 1.00
- For indirect immunofluorescence, 10 4cells were cultured on glass coverslips, stimulated with 10 nM mature TGFβ 1 for 10 minutes, stained as described [ 66 67 ] and viewed using a Zeiss LSM-510 confocal microscope. [W1,W2,W3,W4,W5,I1]: 1.00
- SecB binds portions of the mature secretory protein [ 14 15 16 17 18 ] , and SecA would bind to the SecB-precursor complex by virtue of its ability to bind SecB [ 19 20 ] as well as the preprotein [ 21 22 23 ] . Thus, SecA-mediated targeting of the preprotein to translocase would occur. [W1,W2,W3,W4,I1]: 1.00
- In the absence of an appropriate signal at the plasma membrane, these vesicles serve as storage repositories for mature CT. [W1,W2,W3,W4,I1]: 1.00
- Targeted null Brn3c mutants have no mature hair cells [ 1 2 ] . Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [ 3 ] . Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [ 3 ] . Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [ 1 ] ). However, more detailed quantification by others [ 3 ] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. [W1,W2,W3,W4,W5,I1]: 1.00
- This motif has been detected on several proteins, including laminin, collagen and fibrinogen [ 120 ] . A BLAST search for this sequence in TGFβ 1 revealed a 70% similar motif in two parts of the molecule; one in the LAP (data not shown) and one in the mature TGFβ 1 . In mature TGFβ 1 , the DLXXL motif is freely accessible for interactions on the outside of the molecule. [W1,W2,W3,W4,I1]: 1.00
- The RA synovium appears to contain an increased number of phenotypically mature memory T cells as compared with the blood. [W1,W2,W3,W4,I1]: 1.00
- To establish a situation of chronic IL-1β stimulation as might be encountered in an arthritic joint, experiments were designed like that described for Figure 4, but in this case the source of IL-1β was provided by the addition of dermal fibroblasts genetically modified to constitutively secrete mature human IL-1β. [W1,W2,W3,W4,W5,I1]: 1.00
- Following the 14 day podocyte differentiation protocol on coverslips to establish mature podocytes, wild-type and HIV-1 infected podocytes underwent immunofluorescent detection of cyclin D 1 as follows: coverslips were washed three times with 1 × PBS, fixed in 60% acetone/3. [W1,W2,W3,W4,I1]: 1.00
- 1B), and mature granulosa cells (mg, Fig. [W1,W2,W3,W4,I1]: 1.00
- Sequencing the mature transcripts (spliced mRNA) solves three major problems: first, it permits accurate identification of the 5' and 3' UTRs. [W1,W2,W3,W4,I1]: 1.00
- In summary, these tracing data suggest that mature hair cells are not necessary to direct fiber outgrowth and to maintain some fibers at least up to 6 months in the apex of the cochlea. [W1,W2,W3,W4,W5,I1]: 1.00
- Although the first two miRNA genes were identified through forward genetics, the vast majority of known miRNAs were identified by direct cloning of mature 21-22 nucleotide RNAs, either from size-selected total RNA or from purified miRNP complexes. [W1,W2,W3,W4,I1]: 1.00
- The commercial companies GAO visited achieved success in product development by first achieving a mature, stable design supported by completed engineering drawings during an integration phase and then by demonstrating that the product's design was reliable and critical manufacturing processes required to build it were in control before committing to full production. [W1,W2,W3,W4,I1]: 1.00
- Interestingly, in humans with severe systemic inflammation, very high serum levels of ProCT and its component peptides are accompanied by normal or only slightly increased levels of mature CT [ 17]. [W1,W2,W3,W4,I1]: 1.00
- Joining by entwining also occurs when intact mature macrofibers with loops at their ends meet [ 9 ] . The resulting structures are irregular in diameter along their length reflecting differences in the diameter and/or topology of the two initial partners. [W1,W2,W3,W4,I1]: 1.00
- Secretory maturation toxicity is one possible mechanism for elevated levels of intracellular mature holoAPP and causes retarded conversion of mature holoAPP to sAPP α . This pattern was not observed following statin treatment, excluding maturation toxicity as a mechanism underlying the altered levels of mature cellular holoAPP. [W1,W2,W3,W4,W5,I1]: 1.00
- This motif has been detected on several proteins, including laminin, collagen and fibrinogen [ 120 ] . A BLAST search for this sequence in TGFβ 1 revealed a 70% similar motif in two parts of the molecule; one in the LAP (data not shown) and one in the mature TGFβ 1 . In mature TGFβ 1 , the DLXXL motif is freely accessible for interactions on the outside of the molecule. [W1,W2,W3,W4,I1]: 1.00
- It is the prime virtue of Alan Wolfe's new book, One Nation, After All , that it grasps the contours of what he calls this "mature" middle class. [W1,W2,W3,W4,I1]: 1.00
- The mouse anti-rat LHR monoclonal antibody (mAb), clone 3B5, was developed against purified rat LHR [ 21 ] . The antibody showed immunoreactivity with rat granulosa cells of mature (preovulatory) follicles, ovarian thecal and interstitial cells, granulosa-lutein cells of developing, mature and regressing CL, and with testicular Leydig cells, and no reactivity with rat kidneys [ 22 ] . During the last ten years, affinity purified 3B5 antibody has been used in several immunohistochemical studies [ 23 24 25 26 ] . To our knowledge, however, no analysis of the 3B5 antibody by western blot has been reported. [W1,W2,W3,W4,I1]: 1.00
- Immunohistochemical staining was done using rabbit polyclonal antibodies raised against synthetic peptides that correspond to regions in the mature forms of TGF-β 1 , TGF-β 2 and TGF-β 3 : anti-TGF-β 1 -LC and anti-TGF-β 1 -CC [ 19], anti-TGF-β 2 (sc-90; Santa Cruz Biotechnologies Inc, Santa Cruz, CA, USA), and anti-50-60-β 3 -LC [ 20], respectively. [W1,W2,W3,W4,W5,I1]: 1.00
- Some programs were less mature than others and had not fully implemented all of the practices. [W1,W2,W3,W4,I1]: 1.00
- studied the effect of strain rate on MCL failures in skeletally immature and mature rabbits. [W1,W2,W3,W4,W5,I1]: 1.00
- Portable Network Graphic (PNG) File showing enhanced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (A) or Western blotting after biotinylation of all proteins and streptavidin detection (B). [W1,W2,W3,W4,I1]: 1.00
- military military to make people to go off to the military voluntarily but it's it has helped them and they are just a lot more mature than the average student and [W1,W2,W3,W4,I1]: 1.00
- Having reached this mature configuration, a person continues to pile up grains of experience, building on the existing base. [W1,W2,W3,W4,W5,I1]: 1.00
- The extent of the precisely conserved arm (thus including a potential miRNA) was in most cases longer than the mature product, and it would have been arbitrary to select a candidate miRNA sequence just so that it began with a U. Notably, a recent study suggests that different chemical strategies for capturing miRNA molecules may differentially recover miRNAs according to their 5' ends [ 33 ] , so reevaluation of miRNA 5' bias may be in order. [W1,W2,W3,W4,I1]: 1.00
- Since production of placental hormones is characteristic of mature syncytium [ 5 9 11 53 54 ] , which shows in vivo cytoplasmic ERβ expression only, one may also assume that estrogens stimulate production of placental hormones by ST. [W1,W2,W3,W4,W5,I1]: 1.00
- The evidence that the authors provide for these cells being differentiated includes analysis of gene expression, surface antigens, and immunocytochemistry typical of the mature tissues. [W1,W2,W3,W4,W5,I1]: 1.00
- Since we have shown that artificial removal of the prosequence of HsN3 leads to a blockage of the normal assembly of HsN3 into the mature proteasome (Fig. [W1,W2,W3,W4,I1]: 1.00
- However, strong ERα expression is apparent in early culture (top inset E), and persists thereafter (top inset F), apparently due to the lack of mature syncytium formation. [W1,W2,W3,W4,W5,I1]: 1.00
- it's a really big problem i think and um takes mature people to [W1,W2,W3,W4,I1]: 1.00
- Development of mature ST aggregates (m, panel C) was associated with gradual diminution of nuclear staining (solid > white arrows), but persisting cytoplasmic ERβ immunoreactivity. [W1,W2,W3,W4,I1]: 1.00
- At P12 either endothelial-lined vessels or a more mature SC were present in most sections. [W1,W2,W3,W4,I1]: 1.00
- This antiserum reacts with the CT molecule, whether it is in the free, amidated, 32-amino acid mature form, or within its precursor propeptides [ie procalcitonin, the conjoined calcitonin:calcitonin carboxypeptide-I (CT:CCP-1), or the free immature, unamidated CT]. [W1,W2,W3,W4,I1]: 1.00
- Cells in mature animal tissues are not, as we had thought, irreversibly differentiated. [W1,W2,W3,W4,I1]: 1.00
- Western blot analysis of protein extracts from normal rat ovaries and human and porcine mature CL with 3B5 antibody showed six distinct bands migrating between ~92 and ~48 kDa (lanes 2, 4 and 6, Fig. [W1,W2,W3,W4,I1]: 1.00
- Live Flesh , about a man who accidentally shoots his lover's husband, is deemed the director's "most mature film yet" (Jack Mathews, Newsday ). While the critics like Almodóvar's foray into political commentary--he indicts the Franco regime and Spain's ruling conservative party--they wax most enthusiastic over his signature touch: kinky sex scenes. [W1,W2,W3,W4,I1]: 1.00
- Axenic seedlings, and soil grown plants were maintained in a growth chamber at 23°C under a 16-hour photoperiod until appropriate organs were mature. [W1,W2,W3,W4,I1]: 1.00
- Yet, cell surface staining was observed only on classic LH/CG targets, granulosa and theca cells of preovulatory follicles and mature luteal cells, but also in the vaginal epithelium. [W1,W2,W3,W4,I1]: 1.00
- Genomic DNA was extracted from mature leaves using a miniprep protocol [ 74 ] modified by the addition of a CTAB extraction [ 75 ] . A. thaliana leaves were harvested, hand washed and then ground to a fine powder in liquid nitrogen. [W1,W2,W3,W4,I1]: 1.00
- Dendritic cell precursors (arrows) and mature DC (black arrowheads) were stained. [W1,W2,W3,W4,W5,I1]: 1.00